近日，司法鑒定科學研究院嚴慧課題組湯以綾老師，使用IPHASE品牌產品：II相代謝穩(wěn)定性試劑盒（UGTs）在《Journal of Pharmaceutical and Biomedical Analysis》權-威期刊上發(fā)表文章《Metabolism of dipentylone in zebrafish and human liver microsomes determined by liquid chromatography–high resolution mass spectrometry 》，影響因子3.4！

本論文中，利用LC-HRMS分析dipentylone在斑馬魚和HLM體內和體外的代謝，并成功鑒定了代謝產物及其主要代謝途徑，選擇了合適的代謝物作為生物標志物。

摘要

The consumption of novel psychoactive substances (NPS) is exceedingly prevalent in society, as these substances are sold and distributed as “legal highs.” One novel synthetic cathinone emerging in the market is 1-(1,3-ben-zodioxol-5-yl)–2-(dimethylamino) pentan-1-one (dipentylone). The goal of this work was to study the in vivo and in vitro metabolism of dipentylone in zebrafish and human liver microsomes (HLMs) by liquid chromatography-high resolution mass spectrometry (LCHRMS). The zebrafish and HLM samples contained 14 dipentylone metabolites, specifically 12 phase I metabolites and 2 phase II metabolites. The main metabolic pathways included monohydroxylation (M1 and M2), N-dealkylation (M3), hydroxylation of the aromatic ring and dealkoxylation of M3 (M4), O-dealkylation (M5), N-dealkylation of M5 (M6), reduction of carboxide (M7), monohydroxylation of M5 (M8), dehydrogenation (M9), dealkoxylation (M10), N-dealkylation of M10 (M11), dealkoxylation of M9 (M12), glucuronidation of M5 (M13), and sulfation (M14). The monohydroxylated metabolite (M2) can be recommended as metabolic markers for dipentylone. This study is the first to identify a target compound for monitoring the abuse of dipentylone and to determine the essential chemical structure of the metabolites for urther toxicological research.

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